Screening and Isolation of Extracellular Protease Producing Bacteria from the Maharloo Salt Lake

Authors

  • Aboozar Kazemi Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
  • Alireza Ebrahiminezhad Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
  • Maryam Shahbazi Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
  • Najme Talebnia Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
  • Sara Rasoul-Amini Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
  • Younes Ghasemi Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Abstract:

      Screening and identification of moderately halophilic protease producing bacteria from different regions of Maharloo, a hypersaline lake in the southern area of Iran, were the objectives of this study. In the preliminary screening, 16 isolates exhibited proteolytic activity on saline skim milk agar. All isolates were able to grow comfortably in the media containing 7-15% of salt. Protease activity was assessed through in vitro colorimetric assays for general proteases and the strains were identified by 16S rDNA molecular marker. In comparison to Gram-negative bacteria, the Gram-positive rods, displayed higher proteolytic activities, and Bacillus sp. BCCS 041 was found as the highest protease producing strain with 0.43 U/ml of supernatant activity.

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Journal title

volume 7  issue 3

pages  175- 180

publication date 2011-07-01

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